目的 探讨五味子藤茎提取物(extract from the stems of Schisandra chinensis,SCE)对高脂饮食(high fat diet, HFD)致小鼠肥胖的影响。方法 建立8周高脂饮食诱导的C57BL/6J小鼠肥胖模型。通过检测血清中天冬氨酸氨基转移酶(AST)、丙氨酸氨基转移酶(ALT)、甘油三酯(TG)、总胆固醇(T-CHO)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)及肝组织中甘油三酯(TG)、总胆固醇(T-CHO)、谷胱甘肽(GSH)、丙二醛(MDA)含量,结合H&E和油红O染色观察病理学变化,探讨SCE对肥胖小鼠的影响。此外,采用免疫荧光染色检测细胞色素P4502E1(CYP2E1)、4-羟基壬烯醛(4-HNE),并通过Western blot分析诱导型一氧化氮合酶(iNOS)、诱导型环氧化酶(COX-2)蛋白表达,探讨SCE对肝脏脂质过氧化及炎症反应的影响。结果 与模型组相比,SCE低、高剂量组均明显降低血清中AST、ALT、TG、T-CHO、LDL-C和肝组织中TG、T-CHO、MDA水平,同时升高了血清中HDL-C和肝组织中GSH水平,改善肝组织的脂肪空泡、炎性浸润、脂滴堆积、氧化应激和炎症反应。结论 SCE可能通过抑制氧化应激和炎症反应改善HFD诱导肥胖小鼠的脂代谢。
Abstract
OBJECTIVE To study the effect of extract from the stems of Schisandra chinensis(SCE) on high-fat diet (HFD)induced obesity in mice. METHODS The model of HFD induced obese model of C57BL/6J mice for 8 weeks was established. The contents of serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), triglyceride (TG), total cholesterol (T-CHO), low density lipoprotein cholesterol (LDL-C), high density lipoprotein cholesterol(HDL-C) and hepatic triglyceride (TG), total cholesterol (T-CHO), glutathione (GSH)and malondialdehyde(MDA) were measured. The pathological changes with H&E staining and oil red O was observed for investigating the effect of SCE in the obese mice. In addition, the assay of CYP2E1, 4-HNE using immunofluorescence staining and the expression of iNOS and COX-2 protein with Western blot analyzing was measured to explore the effect of SCE on lipid peroxidation and inflammatory response. RESULTS Compared with the HFD group, low and high dose groups of SCE significantly reduced the serum AST, ALT, TG, T-CHO, LDL-C and hepatic TG, TC and MDA levels, elevated the serum HDL-C and hepatic GSH levels and improved liver tissue steatosis, inflammatory infiltration and accumulation of lipid droplets, oxidative stress and inflammatory response. CONCLUSION SCE may improve lipid metabolism in HFD induced obese mice by inhibiting oxidative stress and inflammatory response.
关键词
五味子藤茎提取物 /
高脂饮食 /
肥胖 /
氧化应激 /
炎症反应
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Key words
extract from the stems of Schisandra chinensis /
high fat diet /
obesity /
oxidative stress /
inflammatory response
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中图分类号：
R965
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参考文献
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脚注
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基金
吉林省科技发展计划资助项目(20160209008YY);吉林农业大学青年拔尖人才支持计划资助项目(2016-2018)
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